Journal: Communications Biology

Article Title: DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice

doi: 10.1038/s42003-023-04643-2

Figure Lengend Snippet: Ldlr − / − and Ldlr − / − Diaph1 − / − male mice were fed Western Diet (WD) for 16 weeks. a Representative images of en face Oil Red O staining of aortas. Quantification of plaque area as percentage of Oil Red O-stained area over total aortic surface area is shown. b – e Representative images of aortic arch sections are shown and quantified for the following: b H&E; c Oil Red O; d CD68; and e , Picrosirius Red. In d , the secondary antibody-alone control is shown. Scale bar: 250 µm. The mean ± SEM is reported. The number of independent mice/group is indicated in the figure as individual data points. Statistical analyses regarding testing for the normality of data followed by appropriate statistical analyses were described in Materials and Methods. P values were determined by unpaired T-test.

Article Snippet: Sections were incubated with propylene glycol for 2 min, then incubated with pre-heated Oil Red O staining solution (American MasterTech, STOROPT) at 60 °C for an additional 8 min.

Techniques: Western Blot, Staining

Journal: Communications Biology

Article Title: DIAPH1 mediates progression of atherosclerosis and regulates hepatic lipid metabolism in mice

doi: 10.1038/s42003-023-04643-2

Figure Lengend Snippet: Ldlr − / − and Ldlr − / − Diaph1 −/− male mice were fed WD for 16 weeks. a Representative immunofluorescence staining and quantification of DIAPH1 in the liver of Ldlr −/− and Ldlr −/− Diaph1 −/− male mice. b Representative images of H&E and Oil Red O staining in liver and quantification is shown. c Quantification of free cholesterol content in liver. d Quantification of total cholesterol content in liver. e Quantification of total liver triglycerides. f Representative images of Picrosirius Red staining in liver and quantification is shown. g Quantification of whole liver weight. The mean ± SEM is reported. The number of independent mice/group is indicated in the figure as individual data points. In a the secondary antibody–alone control is shown. Scale bars: 250 µm, and inset boxes: 50 µm. Statistical analyses regarding testing for the normality of data followed by appropriate statistical analyses were described in Materials and Methods. P- values were determined by unpaired T-test or Wilcoxon rank-sum test depending on if the data passed the Shapiro-Wilk normality test.

Article Snippet: Sections were incubated with propylene glycol for 2 min, then incubated with pre-heated Oil Red O staining solution (American MasterTech, STOROPT) at 60 °C for an additional 8 min.

Techniques: Immunofluorescence, Staining